HAN LAB@NJUCM
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The team of Professor Xin Han from Nanjing University of Traditional Chinese Medicine Advanced Healthcare Materials: Construction of a new platform for tumor stem cell sorting based on microfluidic chip technology and its application in targeted tumor stem cell drug screening
Cancer stem cells are a rare subgroup of cancer cells. They can initiate and promote the formation of tumors due to their self-renewal ability and multi-differentiation potential. Cancer stem cells are resistant to traditional chemotherapy and radiotherapy, and are responsible for the recurrence of many cancers. In-depth study of the molecular characteristics and signal pathways of cancer stem cells not only has important theoretical significance for cancer treatment targeting cancer stem cells, but also has urgent clinical needs. Cancer stem cells have the characteristics of low content, heterogeneity, dynamic change, and lack of specific molecular targets. It is very challenging to use traditional in vitro experimental methods to study. The prediction and analysis of the results of animal experiments are very complicated and difficult. Therefore, how to study the heterogeneity of cancer stem cells and analyze their molecular characteristics and signaling pathways is an urgent need for new high-throughput single cell analysis methods, which have important theoretical significance for discovering new tumor molecular targets and reducing cancer recurrence and metastasis. And practical value.
In response to the above key issues, Professor Han Xin’s team from Nanjing University of Traditional Chinese Medicine School of Integrative Medicine has developed a new label-free tandem microfluidic tumor stem cell sorting technology, which fully considers and utilizes the size and hardness of tumor stem cells. , Adhesion ability and other physical properties, which can achieve more stable, fast and efficient marker-free sorting and enrichment of cancer stem cells; and using this technology platform for targeted drug screening and functional identification of cancer stem cells, successfully derived from licorice A potential drug molecule that can effectively inhibit lung cancer stem cells was screened from natural products. This technology platform provides important tools and means for the discovery of new tumor stem cell molecular targets and targeted drug screening, as well as the development of new tumor suppression strategies for new tumor stem cell targets.
The work "Microfluidic tandem mechanical sorting system for enhanced cancer stem cell isolation and ingredient screening" was selected as the inside back cover and published by Advanced Healthcare Materials. Nanjing University of Traditional Chinese Medicine is the only communication unit. The first author, Professor Han Xin is the corresponding author, and the young lecturer Sun Jia is the co-corresponding author. This research has been supported by the National Natural Science Foundation of China (31901010), the Jiangsu University Advantage Discipline Construction Project (Integrated Traditional Chinese and Western Medicine), and Jiangsu Province Distinguished Professor Natural science funded talent project, Jiangsu Province "Double Innovation Plan" team project, etc.
The above work is another important achievement based on a series of research results of Professor Han Xin's construction of a single cell analysis and sorting platform based on microfluidic chip technology. Professor Han Xin previously developed a novel microfluidic chip cell transfection technology, which successfully overcomes the bottleneck of molecular delivery and gene editing in difficult-to-transfect cells such as T cells and hematopoietic stem cells, and provides new opportunities for the clinical application of tumor immunotherapy and other cell therapy. (Han X, et al. Science Advances, 2015, 1, e1500454; Han X, et al. Advanced Biosystems, 2017, 1, 1600007); developed a high-throughput microfluidic cell sorting chip, combined with CRISPR technology enables rapid and effective screening and identification of tumor molecular targets on a genome-wide scale (Han X, et al. Angewandte Chemie International Edition, 2016, 55, 8561–8565); as the main participant, he developed a series of novel microfluidics Single-cell chips are used for cell manipulation, capture, analysis and heterogeneity research (Zhang K, Han X, et al. JACS, 2014, 136, 10858; Ma Y, Han X, et al. Science Advances, 2018, 4, eaas9274; Zhang P, Han X, et al. Angewandte Chemie International Edition, 2019, 58, 13700−13705). The micro-nano manufacturing and translational medicine cross-team laboratory (https://www.hanlabnj.com/) where Professor Han Xin’s team is located is committed to using a new generation of gene editing technology, novel microfluidic chip technology and multifunctional intelligent biomaterials to solve The key scientific and technical issues in the process of biomedical transformation. The team members have published Science Advances, Angewandte Chemie, Advanced Materials, Advanced Functional Materials, Small, Nano Letters, Biomaterials, Oncogene and other high-level research papers as the first author or corresponding author 10 Multiple articles.
Corresponding author's profile:
Han Xin is a professor at the School of Integrative Medicine, School of Medicine, Nanjing University of Traditional Chinese Medicine, the head of the Department of Biochemistry and Molecular Biology and the subject leader, and a distinguished professor of Jiangsu Province. Mainly engaged in the research of microfluidic chip technology and translational medicine, gene editing and multifunctional bio-nano materials. Representative works include Science Advances, Angewandte Chemie, Small, Advanced Healthcare Materials, Analytical Chemistry, Aging Cell and other domestic and foreign journals published more than 30 SCI papers.